Analytical Instrument Facility

Applied Biosystems 3130 Genetic Analyzers

applied bio
Make : Applied Biosystems
Model : 3130 Genetic Analyzers
Specification : Sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening. 3130 Genetic Analyzer give you all the benefits of the Applied Biosystems suite of fluorescence-based capillary electrophoresis (CE) systems. Capillary electrophoresis (CE) is a versatile and widely used analysis platform with application in diverse areas such as analytical chemistry, chiral separations, clinical, forensics, molecular biology, natural products, organic chemistry, and the pharmaceutical industry. Forensic applications of CE include fragment analysis, DNA sequencing, SNP typing, and analysis of gunshot residues, explosive residues, and drugs.
Fragment analysis is a widely used method for short tandem repeat (STR) profiling for human identification (HID) due to the single-base resolution capability of CE. This approach circumvents the tedious and expensive approach of DNA sequencing for STR typing.

Working Principles

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During capillary electrophoresis, the extension products of the cycle sequencing reaction enter the capillary as a result of electrokinetic injection. A high voltage charge applied to the buffered sequencing reaction forces the negatively charged fragments into the capillaries. The extension products are separated by size based on their total charge. The electrophoretic mobility of the sample can be affected by the run conditions: the buffer type, concentration, and pH, the run temperature, the amount of voltage applied, and the type of polymer used. Shortly before reaching the positive electrode, the fluorescently labeled DNA fragments, separated by size, move across the path of a laser beam. The laser beam causes the dyes on the fragments to fluoresce. An optical detection device on Applied Biosystems genetic analyzers detects the fluorescence The Data Collection Software converts the fluorescence signal to digital data, then records the data in a file.

Applications

  • SNP screening,
  • DNA sequencing
  • SNP validation

User Instructions

  1. Sample must be pure and all contaminants must be removed.
  2. Sample should be in multiple of 96 in ABI Sequencing Plate

Contact Us

Contact: 0542-2635247
Email Id: directoriivr@gmail.com

Genetic Analyzers Charges includes all charges for DNA Sequencing

ICAR Institutes UGC Recognized Universities Other National Lab/R&D's Industry
850/- per sample 850/- per sample 850/- per sample 1000 /- per sample
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